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245	1	0	_aMedium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures1
490	0		_vCanadian Journal of Botany, 50(1), p.199-204, 1972
520	3		_aThe composition of a medium is described that proved useful to culture callus of a variety of monocotyledonous and dicotyledonous plants. Growth on the medium was often better than on some other excellent media. In addition to supporting rapid cell growth, a soft, friable type of colony growth was often obtained. This type of loose, friable cell growth facilitated work with single cells and the enzymatic removal of cell walls in related studies. A high level of auxin-type growth-regulating substances (AGRS)generally favored cell cultures of n~onocotyledonous plants, while low levels of cytokinin were essential for most dicotyledonous cell cultures. Some cultures of dicotyledonous plant cells adapted to a cytokininfree medium containing a high level of 2,4-dichlorophenoxyacetica cid (2,4-D)orp-chlorophenoxyacetic acid CpCPA). The preferred AGRS were 2,4-D and pCPA. Citrate, succinate, and 2(N-morpholino)ethane sulfonic acid (MES)were effective medium buffers, but phosphate alone seemed adequate to buffer the medium at pH 5.8-5.9.
700	1	2	_aSchenkan, R.U.
700	1	2	_aHildebrandt, D.A.C.
856	4	0	_uhttps://drive.google.com/file/d/1LRRtyo1HLlxnhXirL7u1BHrlG0NOr2v1/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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