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090			_aB-13461
245	1	0	_aSecretion-and-capture cell-surface display for selection of target-binding proteins
490	0		_vProtein Engineering, Design & Selection, 24(6), p.525-530, 2011
520	3		_aThis report describes a new cell-surface display system, the Secretion and Capture Technology (SECANTTM)platform, which relies on in vivo biotinylation of the protein of interest followed by its capture on the avidinated surface of the parent cell. Cell sorting techniques are then used to isolate clones that display target-binding protein. A distinguishing feature of this method is its ability to display complex proteins, such as full-length immunoglobulin G (IgG)antibodies, on living cells. In this proof-of-concept study, Saccharomyces cerevisiae cells that displayed Herceptin IgG were isolated from a 10 000-fold excess of cells that displayed a lysozymebinding antibody.
650	1	4	_aANTIBODY DISPLAY
650	1	4	_aIGG DISPLAY
650	1	4	_aPROTEIN ENGINEERING
650	1	4	_aSECRETION AND CAPTURE
650	1	4	_aYEAST DISPLAY
700	1	2	_aRakestraw, J.A.
700	1	2	_aAird, D.
700	1	2	_aAha, P.M.
700	1	2	_aBaynes, B.M.
700	1	2	_aLipovsek, D.
856	4	0	_uhttps://drive.google.com/file/d/1CCjuFYd6clarK3jHTWeArYd10IPMrb5C/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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