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245	1	0	_aIn vitro construction of a human blood vessel from cultured vascular cells: A orphologic study
490	0		_vJournal of Vascular Surgery, 17(3), p.499-509, 1993
520	3		_aThe blood vessel equivalent was constructed with the three following human cell types: vascular smooth muscle cells, endothelial cells, and fibroblasts. A tissuelike structure was obtained from the contraction of a tubular collagen gel (human origin)by vascular smooth muscle cells, which created a media-like structure. An adventia-like tissue was added around the media-like structure by embedding fibroblasts into a collagen gel. An endothelium was established within the tubular structure after intrahuninal cell seeding. Resdts: CelJ orientation and gel contraction were followed up over time. Vascular smooth muscle cells developed a complex tridhnensional network and were oriented in a circular fashion around the tube's axis. In contrast, fibroblasts were randomly oriented. A viable, homogeneous, and well-characterized endothelium was observed. These endothelial cells showed a slightly elongated structure and were oriented parallel to this vascular equivalent axis.
700	1	2	_aL'Heureux, N.
700	1	2	_aGermain, L.
700	1	2	_aLabbe, R.
700	1	2	_aAuger, F.A.
856	4	0	_uhttps://drive.google.com/file/d/1_1eHwCF5DDlDZCp3tv7cOLqYkznoOYiS/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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