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090			_aB-13506
245	1	0	_aAvailable methods for assembling expression cassettes for synthetic biology
490	0		_vAppl. Microbiol. Biotechnol., 93(5), p.1853-1863, 2012
520	3		_aStudies in the structural biology of the multicomponent protein complex, metabolic engineering, and synthetic biology frequently rely on the efficient over-expression of these subunits or enzymes in the same cell. As a first step, constructing the multiple expression cassettes will be a complicated and time-consuming job if the classic and onventional digestion and ligation based cloning method is used. Some more efficient methods have been developed, including (1)the employment of a multiple compatible plasmid expression system, (2)the rare-cutter-based design of vectors, (3)in vitro recombination (sequence and ligation independent cloning, the isothermally enzymatic assembly of DNA molecules in a single reaction), and (4)in vivo recombination using recombination-efficient yeast (in vivo assembly of overlapping fragments, reiterative recombination for the chromosome integration of foreign expression cassettes). In this review, we systematically introduce these available methods.
650	1	4	_aSYNTHETIC BIOLOGY
650	1	4	_aSIMULTANEOUS EXPRESSION
650	1	4	_aPATHWAY CONSTRUCTION
650	1	4	_aYEAST RECOMBINATION
650	1	4	_aCRE-LOXP SITESPECIFIC
650	1	4	_aRECOMBINATION
650	1	4	_aACEMBL SYSTEM
700	1	2	_aWang, T.
700	1	2	_aMa, X.
700	1	2	_aZhu, H.
700	1	2	_aLi, A.
700	1	2	_aDu, G.
700	1	2	_aChen, J.
856	4	0	_uhttps://drive.google.com/file/d/1iDipvGZwCXAnfZSq2I7vtolbEgKTddh_/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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