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090			_aB-13565
245	1	0	_aPCR-amplification of GC-rich regions: 'slowdown PCR'
490	0		_vNature Protocols, 3(8), p.1312-1317, 2008
520	3		_aThe polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483
700	1	2	_aFrey, U.H.
700	1	2	_aBachmann, H.S.
700	1	2	_aPeters, J.
700	1	2	_aSiffert, W.
856	4	0	_uhttps://drive.google.com/file/d/1sRGI3fOEhk7PMmmc5kvZwguaoskiX4Mk/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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