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090 _aB-13577
245 1 0 _aSeparation of glutaraldehyde and some of its aldol condensation products by hydroxyl-aldehyde group affinity chromatography
490 0 _vJournal of BioChemical and Biophysical Methods, 22(4), p.321-329, 1991
520 3 _aGlutaraldeh)dc exists in aqueous solution in equilibrium with monomers and polymers of cyclic and open-chain hemialdals and hydrates. At alkaline pH oligomeric and polymeric a,fl-unsaturated aldehyde derivatives are formed from primarily produced aldol condensation products. This communication reports a method for separation of such aldol condensates by means of a new high performance liquid chromatography technique based on the affinity of aldehyde groups for hydroxyl groups of a hydroxylated polyether matrix (Bio-Gel SEC-10). Five peaks corresponding to different aldol condensates of glutaraldehyde were obtained from the affinity column. They have been distinguished by ~tt-NMR and UV spectroscopy. Kinetic measurements yielded formation rates for the different aldol condensates.
650 1 4 _aGLUTARALDEHYDE
650 1 4 _aCONDENSATION PRODUCT
650 1 4 _aOLIGOMERIC
650 1 4 _aPOLYMERIC
650 1 4 _aSEPARATION
650 1 4 _aPARTIAL CHARACTERIZATION
650 1 4 _aHYDROXYL-ALDEHYDE GROUP
650 1 4 _aINTERACTION CHROMATOGRAPHY
700 1 2 _aHolmquist, L.
700 1 2 _aLewin, M.
856 4 0 _uhttps://drive.google.com/file/d/1ONw-8U-l0CTy_suFyU51SeF046_tt9g3/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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