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245 1 0 _aRecovery of l-(+)-lactic acid by anion exchange resin Amberlite IRA-400
490 0 _vBioChemical Engineering Journal, 11(2-3), p.189-196, 2002
520 3 _aAmberlite IRA-400 anion exchange resin was applied for the recovery of l-(+)-lactic acid from fermentation broth. Adsorption isotherm and breakthrough curves for the separation of l-(+)-lactic acid were obtained at pH 5.0 and 2.0, respectively. Different types of adsorption isotherms were found at pH above and below the pKa (3.86)of lactic acid. The isotherm was found to be a Langmuir type at pH 5.0, whereas t e isotherm was type II (multilayer adsorption)at pH 2.0. At pH 5.0, the maximum adsorption capacity of the resin, qm and dissociation constant,Kd were 222.46 mg/g wet resin and 60.7 mg/ml, respectively. Breakthrough curve for the separation of lactic acid from fermentation brothwas also obtained. The maximum adsorption capacity (197.09 mg/g wet resin)at pH 5.0wasmuch higher than that at pH 2.0 (106 mg/g wet resin). Proper elution and washing conditions were sought by using H2SO4, methanol, ammonia or their mixtures as eluant. When column separation was performed at pH 5.0 by using 50
650 1 4 _aADSORPTION
650 1 4 _aLACTIC ACID
650 1 4 _aSEPARATION
650 1 4 _aANION ION EXCHANGE RESIN
650 1 4 _aIRA-400
700 1 2 _aCao, X.
700 1 2 _aShik Yun, H.
700 1 2 _aKoo, Y.
856 4 0 _uhttps://drive.google.com/file/d/15CLzbGD1J3-jA-tQWy2BJ5DQo-H04UKf/view?usp=drivesdk
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