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| 003 | MX-MdCICY | ||
| 005 | 20250625153932.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-13930 | ||
| 245 | 1 | 0 | _aDetermination of indole-3-pyruvic acid levels in Arabidopsis thaliana by gas chromatography-selected ion monitoring-mass spectrometry |
| 490 | 0 | _vJournal of Chromatography A, 800(1), p.101-108, 1998 | |
| 520 | 3 | _aA rapid and simple method is described for the determination of indole-3-pyruvic acid (IPA)levels in Arabidopsis thaliana by gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS). The method includes derivatization of IPA with hydroxylamine in the crude extract, followed by ethyl acetate partitioning, solid-phase extraction with C resin, reversed-phase high-performance liquid chromatography (HPLC), and GC-SIM-MS. Three derivatizing 18 reagents were tested; these were entafluorobenzylhydroxylamine, pentafluorophenylhydrazine, and hydroxylamine. Hydroxylamine proved to be the most useful, as the IPA-oxime was easiest to purify from plant extracts and was the most stable. IPA was quantified in Arabidopsis seedlings ranging in age from 5 to 12 days; levels varied from 4 to 13 ng/ g, peaking at 7-9 days. | |
| 650 | 1 | 4 | _aARABIDOPSIS THALIANA |
| 650 | 1 | 4 | _aDERIVATIZATION, GC |
| 650 | 1 | 4 | _aINDOLEPYRUVIC ACID |
| 650 | 1 | 4 | _aPENTAFLUOROPHENYLHYDRAZINE |
| 650 | 1 | 4 | _aPENTAFLUOROBENZYLHYDROXYLAMINE |
| 700 | 1 | 2 | _aTam, Y.Y. |
| 700 | 1 | 2 | _aNormanly, J. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1P0PNyPuM9oprfio3F9dBIN_BNzb2hKd3/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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