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090			_aB-14136
245	1	0	_aSubtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis
490	0		_vGenetic Analysis: BioMolecular Engineering, 13(2), p.49-58, 1996
520	3		_aThe present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtrac.tion are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, ]993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.
650	1	4	_aSUBTRACTIVE HYBRIDIZATION
650	1	4	_aENRICHMENT
650	1	4	_aKINETICS
700	1	2	_aErmolaeva, O.D.
700	1	2	_aSverdlov, E.D.
856	4	0	_uhttps://drive.google.com/file/d/1BDeec3jdMNSyZ5OXU_tTk-O1zdhvT6fD/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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