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090			_aB-14168
245	1	0	_aA fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123
490	0		_vJournal of Immunological Methods, 131(2), p.269-275, 1990
520	3		_aAnalysis of the functional activity of phagocytes is of great importance in the differential diagnosis of patients with recurrent bacterial infections. Here we describe a method to determine the production of reactive oxygen intermediates (ROI)by microcytofluorometry using dihydrorhodamine 123, a derivative of rhodamine 123. Using this method the ROI production of erythrocyte-depleted whole blood samples can ::,e measured without further time-consuming purification steps. Possible harmful manipulation of the isolated cells can also be avoided and highly reproducible and significant results are obtained in the minimum of time. This assay provides a very sensitive alternative to the clinically used NBT test in the diagnosis of patients with chronic granulomatous disease (CGD). Moreover, the analysis of oxygen-dependent effector functions of murine effector cells and cell lines may be important in investigating resistance to certain microbes (e.g., Candida albicans, Staphylococcus aureus or different protozoa such as Toxoplasma gondii or Leishmania species).
650	1	4	_aDIHYDRORHODAMINE 123
650	1	4	_aFLOW MICROCYTOFLUOROMETRY
650	1	4	_aREACTIVE OXYGEN INTERMEDIATE
650	1	4	_aPHAGOCYTE
650	1	4	_aCHRONIC GRANULOMATOUS DISEASE
700	1	2	_aEmmendösrffer, A.
700	1	2	_aHecht, M.
700	1	2	_aLohmann-Matthes, M-L.
700	1	2	_aRoesler, J.
856	4	0	_uhttps://drive.google.com/file/d/1B9JpUljjAiWPfbDqIapjTs3U25zmjqLH/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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