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090			_aB-14249
245	1	0	_aMAP Kinase Phosphorylation and cAMP Assessment in Fungi
490	0		_vMethods in Molecular Biology, 835, p.571-583, 2012
520	3		_aThe cyclic AMP (cAMP)signaling and mitogen-activated protein (MAP)kinase pathways are the most important signal transduction pathways in eukaryotes. In many plant pathogenic fungi they play pivotal roles in virulence and development. Identifi cation and understanding the role of signal transduction pathways in regulation of cellular responses require robust biochemical techniques. Determination of both the phosphorylation status of MAPKs and the intracellular levels of cAMP is required to unravel the function of these pathways during adaptation of fungi to environmental stress conditions or when particular fungal genes are disrupted or silenced. Here we describe protocols to determine the phosphorylation status of three different MAPKs including Fus3, Slt2 and Hog1 as well as a protocol to measure the intracellular levels of cAMP levels. These protocols can be adapted for a wide range of fungi.
650	1	4	_aCAMP MEASUREMENT
650	1	4	_aMITOGEN-ACTIVATED PROTEIN KINASE
650	1	4	_aPROTEIN KINASE A
650	1	4	_aPHOSPHORYLATION
650	1	4	_aFUS3
650	1	4	_aSLT2
650	1	4	_aHOG1
700	1	2	_aMehrabi, R.
700	1	2	_aM'Barek, S.B.
700	1	2	_aSaidi, A.
700	1	2	_aAbrinbana, M.
700	1	2	_aDe Wit, P.J.G.M.
700	1	2	_aKema, G.H.J.
856	4	0	_uhttps://drive.google.com/file/d/1hDFDavhdoRGdZNfxSSGrj-6dLeI28E3z/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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