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090			_aB-14411
245	1	0	_aReal-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds
490	0		_vJournal of Virological Methods, 171(1), p.292-294, 2011
520	3		_aMaize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.
650	1	4	_aMAIZE CHLOROTIC MOTTLE VIRUS
650	1	4	_aREAL-TIME RT-PCR
650	1	4	_aQUARANTINE VIRUS
700	1	2	_aZhang, Yongjiang
700	1	2	_aZhao, Wenjun
700	1	2	_aLi, Mingfu
700	1	2	_aChen, Hongjun
700	1	2	_aZhu, Shuifang
856	4	0	_uhttps://drive.google.com/file/d/1Hks_oBYSQEIv2TZW_DCAGdQLPxd16zdu/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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