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003			MX-MdCICY
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040			_cCICY
090			_aB-14611
245	1	0	_aApplication of universally amplifying plastid primers to environmental sampling of a stream periphyton community
490	0		_vMolecular Ecology Resources, 8(5), p.1011-1014, 2008
520	3		_aTo demonstrate the utility of universal plastid primers for probing of environmental samples, we extracted DNA from a tropical stream periphyton community and created two environmental clone libraries. We demonstrate the recovery of DNA sequences corresponding to the major groups of algae observed microscopically in the sample, illustrating the utility of these primers for analysis of environmental samples. Using a touchdown polymerase chain reaction technique, almost 99 percent of recovered sequences correspond to plastid-containing or cyanobacterial taxa, which allows algae to be targeted to the almost complete exclusion of noncyanobacterial prokaryotes and nonplastid-containing eukaryotes.
650	1	4	_a23S RRNA PLASTID MARKER
650	1	4	_aALGAE
650	1	4	_aENVIRONMENTAL SAMPLING
650	1	4	_aPERIPHYTON
650	1	4	_aSTREAM
650	1	4	_aUNIVERSAL PRIMERS
700	1	2	_aSherwood, A.R.
700	1	2	_aChan, Y.L.
700	1	2	_aPresting, G.G.
856	4	0	_uhttps://drive.google.com/file/d/1YiGUDn1d2EozJn6ZHW4CgbxrVcZZyl3L/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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