| 000 | 01699nam a2200337Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625153945.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-14660 | ||
| 245 | 1 | 0 | _aThree Methods for the Introduction of Foreign DNA intoAgrobacterium |
| 490 | 0 | _vMethods in Molecular Biology, 343, p.43-53, 2009 | |
| 520 | 3 | _aThe genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating. | |
| 650 | 1 | 4 | _aELECTROPORATION |
| 650 | 1 | 4 | _aFREEZE/THAW |
| 650 | 1 | 4 | _aTRIPARENTAL MATING |
| 650 | 1 | 4 | _aCONJUGATION |
| 650 | 1 | 4 | _aINCOM-PATIBILITY GROUP |
| 650 | 1 | 4 | _aSELECTIVE PLATES |
| 650 | 1 | 4 | _aCOUNTERSELECTION |
| 650 | 1 | 4 | _aDONOR |
| 650 | 1 | 4 | _aRECIPIENT |
| 650 | 1 | 4 | _aTRANSCONJUGANT |
| 650 | 1 | 4 | _aSELF-TRANSMISSIBLE PLASMID |
| 650 | 1 | 4 | _aORIGIN OF TRANSFER (ORIT). |
| 700 | 1 | 2 | _aWise, A.A. |
| 700 | 1 | 2 | _aLiu, Z. |
| 700 | 1 | 2 | _aBinns, A.N. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/19roaQ8d2vcliIUkWpFdlISlqf3bhTh0A/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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