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090			_aB-14696
245	1	0	_aCloning of Small RNAs for the Discovery of Novel MicroRNAs in Plants
490	0		_vRice Protocols , 109-118, 2013
520	3		_aEndogenous small RNAs can be grouped into several distinct classes of 21-nt-long microRNAs (miRNAs), short interfering RNAs (siRNAs), trans-acting siRNAs (tasiRNAs), and 24-nt long heterochromatic siRNAs. miRNAs are increasingly being recognized as signi fi cant effectors of gene regulation in a wide range of organisms. These molecules are typically 21-nt long and are amenable for cloning by streamlined protocols. Here we detail the methodology for cloning small RNAs in rice to identify novel miRNAs and other important small RNAs. Brie fl y, small RNA molecules are size fractionated, attached to adaptors, and subsequently converted into cDNA and PCR ampli fi ed. Current high-throughput sequencing technologies allow sequencing of the PCR products directly.
650	1	4	_aMCIRORNAS
650	1	4	_aSMALL RNAS
650	1	4	_aPOSTTRANSCRIPTIONAL GENE REGULATION
650	1	4	_aRICE
700	1	2	_aJagadeeswaran, G.
700	1	2	_aSunkar, R.
856	4	0	_uhttps://drive.google.com/file/d/1ZnV9plj52Ids9VnbjTCxCwOLxX5jWbyZ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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