| 000 | 01895nam a2200301Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625153947.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-14755 | ||
| 245 | 1 | 0 | _aStudy and Development of Multilayer Needle-type Enzyme-based Glucose Microsensors |
| 490 | 0 | _vBiosensors, 4(1), p.27-40, 1989 | |
| 520 | 3 | _aGlucose oxidase (GOD)was covalently coupled to a cellulose acetate (CA)layer, using bovine serum albumin (BSA)and parabenzoquinone (PBQ)linkages, Prior to GOD coupling this CA layer was deposited on the platinum tip of a needle-type sensor and covered with an outer layer of polyurethane (PU). Such microsensors were found to be active, their GOD load reaching 1.6 to 3.0 pg mm-' and their glucose response reaching I to 3 PA M-' mme2, even when the upper limit of their linear range reached IO-30 mM. Due to the multilayer structure and composition of these micro- sensors, small anions such as ascorbate were partially discriminated from neutral molecules such as hydrogen peroxide. When implanted sub- cutaneously in anaesthetized rats, sensor responses correlated correctly with blood glucose concentration but presented sensitivity coejjicients signifi- cantly different to those determined in vitro: a 2 point calibration procedure was found necessary for in vivo experiments. | |
| 650 | 1 | 4 | _aGLUCOSE |
| 650 | 1 | 4 | _aMICROSENSOR |
| 650 | 1 | 4 | _aNEEDLE |
| 650 | 1 | 4 | _aSUBCUTANEOUS |
| 650 | 1 | 4 | _aCELLULOSE ACE-TATE |
| 650 | 1 | 4 | _aPOLYURETHANE |
| 650 | 1 | 4 | _aGLUCOSE OXIDASE |
| 650 | 1 | 4 | _aPARABENZOQUIONE |
| 700 | 1 | 2 | _aStemberg, R. |
| 700 | 1 | 2 | _aBarrau, M-B. |
| 700 | 1 | 2 | _aGangiotti, L. |
| 700 | 1 | 2 | _aThévenot, D.R. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1GfelgOp5Tnx2EfXG9LyUS0HeJjEzPlbh/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 |
_2Loc _cREF1 |
||
| 008 | 250602s9999 xx |||||s2 |||| ||und|d | ||
| 999 |
_c48940 _d48940 |
||