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090			_aB-14772
245	1	0	_aA Species-Specific Polymerase Chain Reaction Assay for Rapid and Sensitive Detection of Colletotrichum capsici
490	0		_vMol. Biotechnol, 49, p.48-55, 2011
520	3		_aColletotrichum capsici is an important fungal species that causes anthracnose in many genera of plants causing severe economic losses worldwide. A primer set was designed based on the sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2)regions for use in a conventional PCR assay. The primer set (CcapF/ CcapR)amplified a single product of 394 bp with DNA extracted from 20 Mexican isolates of C. capsici. The specificity of primers was confirmed by the absence of amplified product with DNA of four other Colletotrichum species and eleven different fungal genera. This primer set is capable of amplifying only C. capsici from different contaminated tissues or fungal structures, thereby facilitating rapid diagnoses as there is no need to isolate and cultivate the fungus in order to identify it. The sensitivity of detection with this PCR method was 10 pg of genomic DNA from the pathogen. This is the first report of a C. capsici-specific primer set. It allows rapid pathogen detection and provides growers with a powerful tool for a rational selection of fungicides to control anthracnose in different crops and in the post-harvest stage.
650	1	4	_aCOLLETORICHUM CAPSICI
650	1	4	_aDIAGNOSTICSITS SEQUENCES
650	1	4	_aANTHRACNOSE
650	1	4	_aMOLECULAR DETECTION
700	1	2	_aTorres-Calzada,C.
700	1	2	_aTapia-Tussell, R.
700	1	2	_aQuijano-Ramayo, A.
700	1	2	_aMartin-Mex, R.
700	1	2	_aRojas-Herrera, R.
700	1	2	_aHiguera-Ciapara, I.
700	1	2	_aPerez-Brito, D.
856	4	0	_uhttps://drive.google.com/file/d/1DwcwITyR8EMiI27-aCKjh4XD6xNBw6qJ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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