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245 1 0 _aHeterologous Expression Systems for Lipases: A Review
490 0 _vMethods in Molecular Biology, 861, p.161-178, 2012
520 3 _aThe production of heterologous lipases is one of the most promising strategies to increase the productivity of the bioprocesses and to reduce costs, with the final objective that more industrial lipase applications could be implemented. In this chapter, an overview of the most common microbial expression systems for the overproduction of microbial lipases is presented. Prokaryotic system as Escherichia coli and eukaryotic systems as Saccharomyces cerevisiae and Pichia pastoris are analyzed and compared in terms of productivity, opera-tional, and downstream processing facilities. Finally, an overview of heterologous Candida rugosa and Rhizopus oryzae lipases, two of the most common lipases used in biocatalysis, is presented. In both cases, P. pastoris has been shown as the most promising host system.
650 1 4 _aMICROBIAL LIPASES
650 1 4 _aHETEROLOGOUS EXPRESSION
650 1 4 _aCANDIDA RUGOSA
650 1 4 _aRHIZOPUS ORYZAE
650 1 4 _aESCHERICHIA COLI
650 1 4 _aSACCHAROMYCES CEREVISIAE
650 1 4 _aPICHIA PASTORIS
700 1 2 _aValero, F.
856 4 0 _uhttps://drive.google.com/file/d/1DDnkzEBglDi48WFmaTPXBVUtqK-zQ2je/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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