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245	1	0	_aThree manganese oxide-rich marine sediments harbor similar communities of acetate-oxidizing manganese-reducing bacteria
490	0		_vThe ISME Journal, 6(1), p.2078-2090, 2012
520	3		_aDissimilatory manganese reduction dominates anaerobic carbon oxidation in marine sediments with high manganese oxide concentrations, but the microorganisms responsible for this process are largely unknown. In this study, the acetate-utilizing manganese-reducing microbiota in geographi-cally well-separated, manganese oxide-rich sediments from Gullmar Fjord (Sweden), Skagerrak (Norway)and Ulleung Basin (Korea)were analyzed by 16S rRNA-stable isotope probing (SIP). Manganese reduction was the prevailing terminal electron-accepting process in anoxic incubations of surface sediments, and even the addition of acetate stimulated neither iron nor sulfate reduction. The three geographically distinct sediments harbored surprisingly similar communities of acetate-utilizing manganese-reducing bacteria: 16S rRNA of members of the genera Colwelliaand Arcobacterand of novel genera within theOceanospirillaceaeandAlteromonadaleswere detected in heavy RNA-SIP fractions from these three sediments. Most probable number (MPN)analysis yielded up to 10 6acetate-utilizing manganese-reducing cells cm 3 in Gullmar Fjord sediment. A 16S rRNA gene clone library that was established from the highest MPN dilutions was dominated by sequences ofColwelliaandArcobacterspecies and members of he Oceanospirillaceae, supporting the obtained RNA-SIP results. In conclusion, these findings strongly suggest that (i)acetate-dependent manganese reduction in manganese oxide-rich sediments is catalyzed by members of taxa (Arcobacter, Colwellia and Oceanospirillaceae)previously not known to possess this physiological function, (ii)similar acetate-utilizing manganese reducers thrive in geographically distinct regions and (iii)the identified manganese reducers differ greatly from the extensively explored iron reducers in marine sediments.
650	1	4	_a16S RRNA-SIP
650	1	4	_aMANGANESE REDUCTION
650	1	4	_aMARINE SEDIMENT
650	1	4	_aMPN COUNTS
700	1	2	_aVandieken, V.
700	1	2	_aPester, M.
700	1	2	_aFinke, N.
700	1	2	_aHyun, J.
700	1	2	_aFriedrich, M.W.
700	1	2	_aLoy, A.
700	1	2	_aThamdrup, B.
856	4	0	_uhttps://drive.google.com/file/d/18UFq7GfafOcJOq7pfp5kpYeZQEKSG_jH/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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