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245	1	0	_aNovel Microbial Diversity Retrieved by Autonomous Robotic Exploration of the World's Deepest Vertical Phreatic Sinkhole
490	0		_vASTROBIOLOGY, 10(2), p.201-213, 2010
520	3		_aThe deep phreatic thermal explorer (DEPTHX)is an autonomous underwater vehicle designed to navigate an unexplored environment, generate high-resolution three-dimensional (3-D)maps, collect biological samples based on an autonomous sampling decision, and return to its origin. In the spring of 2007, DEPTHX was deployed in Zacato´n, a deep (*318m), limestone, phreatic sinkhole (cenote)in northeastern Mexico. As DEPTHX descended, it generated a 3-D map based on the processing of range data from54 onboard sonars. The vehicle collected water column samples and wall biomat samples throughout the depth profile of the cenote. Post-expedition sample analysis via comparative analysis of 16S rRNA gene sequences revealed a wealth of microbial diversity. Tradi-tional Sanger gene sequencing combined with a barcoded-amplicon pyrosequencing approach revealed novel, phylum-level lineages from the domains Bacteria and Archaea; in addition, several novel subphylum lineages were also identified. Overall, DEPTHX successfully navigated and mapped Zacato´n, and collected biological samples based on an autonomous decision, which revealed novel microbial diversity in a previously unexplored environment.
650	1	4	_aAUTONOMOUS
650	1	4	_aROBOTICS
650	1	4	_aMICROBIAL DIVERSITY
650	1	4	_a16S RRNA. ASTROBIOLOGY
700	1	2	_aSahl, J.W.
700	1	2	_aFairfield, N.
700	1	2	_aHarris, J.K.
700	1	2	_aWettergreen, D.
700	1	2	_aStone, W.V.
700	1	2	_aSpear, J.R.
856	4	0	_uhttps://drive.google.com/file/d/1p6zAzYfw5MMNIsGzxENV571m7mJajHBP/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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