000			02986nam a2200277Ia 4500
003			MX-MdCICY
005			20250625153950.0
040			_cCICY
090			_aB-14896
245	1	0	_aEvaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitrocultures
490	0		_vAustralian Joournal of Crop Science, 6(1), p.23-30, 2012
520	3		_aHomozygous plants can be obtained in a single generation using doubled haploid technology, which can tremendously facilitate breeding programs. The present study reports on in vitroregeneration and production of double haploid plants through anther and ovule cultures resulting from the hybridization of cultivated species of Cucurbita pepoL. with three other Cucurbita species i.e. C. moschataL., C. ficifoliaand C. martineziiL. Among six sucrose-2,4-Dichlorophenoxyacetic acid (2,4-D)combinations tested, the addition of 90 g l -1 sucrose and 1 mg l -1 2,4-D into the medium was found to be more suitable for callus regeneration from anthers. Anthers from the hybrid C. pepoL. (Queen F 1)× C. moschataL. regenerated the highest percentage (40 percent)of plantlets from callus and highest average number of plantlets per callus (7.5)when the culture medium was supplemented withthe aforementioned concentrations of sucrose and 2,4-D. Ovules collected from the hybrid C. pepoL. (MHTC77 F 1)× C. moschataL. produced the highest percentage of regenerated plantlets (25.33 percent), the highest percentage of responded ovules (18.67 percent)and the highest average number of plantlets per ovule (3.37). Cold pretreatment had negative effects on gynogenesis with the exception of the hybrid C. pepo L. (MHTC77 F 1)× C. moschataL. Chromosome counting in the root tips of androgenic plantlets revealed 50 percent of haploid and (n= x = 20)and 50 percent of dihaploid plantlets (2n= 2x= 40). The assessment of root tips from gynogenic plantlets resulted in 60 percent haploid (2n= x = 20)and 40 percent diploid (2n= 2x= 40)plants. The androgenic strains were evaluated for their vegetative and reproductive traits compared with open local cultivar C. pepoL. (Eskandarani cv.). While the majority of regenerated strains were intermediate between the cultivated species of C. pepoL. and other Cucurbita species, two regenerated strains proved superior to the local cultivar 'Eskandarani' in number of fruits (early and total yield/plot)as well as in total fruit yield per plot.
650	1	4	_aANTHER CULTURE
650	1	4	_aCUCURBITACEAE
650	1	4	_aGENOTYPE
650	1	4	_aHAPLOID
650	1	4	_aOVULE CULTURE
700	1	2	_aRakha, M.T.
700	1	2	_aMetwally, E.I.
700	1	2	_aMoustafa,S.A.
700	1	2	_aEtman, A.A.
700	1	2	_aDewir, Y.H.
856	4	0	_uhttps://drive.google.com/file/d/1WB3NuV11DLJmyrF1H11_t4QjoBtNTfP4/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c49080 _d49080