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245	1	0	_aReplication and trafficking of a plant virus are coupled at the entrances of plasmodesmata
490	0		_vJournal of Cell Biology, 201(7), p.981-995, 2013
520	3		_alant viruses use movement proteins (MPs)to modify intercellular pores called plasmodesmata (PD)to cross the plant cell wall. Many viruses encode a conserved set of three MPs, known as the triple gene block (TGB), typified by Potato virus X (PVX). In this paper, using live-cell imaging of viral RNA (vRNA)and virus-encoded proteins, we show that the TGB proteins have distinct functions during movement. TGB2 and TGB3 established endoplasmic reticulum-derived mem-branous caps at PD orifices. These caps harbored the PVX replicase and nonencapsidated vRNA and repre-sented PD-anchored viral replication sites. TGB1 medi-ated insertion of the viral coat protein into PD, probably by its interaction with the 5?end of nascent virions, and was recruited to PD by the TGB2/3 complex. We pro-pose a new model of plant virus movement, which we term coreplicational insertion, in which MPs function to compartmentalize replication complexes at PD for local-ized RNA synthesis and directional trafficking of the virus between cells.
700	1	2	_aTilsner, J.
700	1	2	_aLinnik, O.
700	1	2	_aLouveaux, M.
700	1	2	_aRoberts, I.M.
700	1	2	_aChapman, S.N.
700	1	2	_aOparka, K.J.
856	4	0	_uhttps://drive.google.com/file/d/1Pe1yfLtetAbL8h7y5SJHAcf25jB2Ox0b/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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