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090			_aB-14911
245	1	0	_aA Plant Virus Movement Protein Forms Ringlike Complexes with the Major Nucleolar Protein, Fibrillarin, In Vitro
490	0		_vJ. Mol. Biol., 376, p.932-937, 2008
520	3		_aFibrillarin, one of the major proteins of the nucleolus, has methyltransferase activity directing 2'-O-ribose methylation of rRNA and snRNAs and is required for rRNA processing. The ability of the plant umbravirus, groundnut rosette virus, to move long distances through the phloem, the specialized plant vascular system, has been shown to strictly depend on the interaction of one of its proteins, the ORF3 protein (protein encoded by open reading frame 3), with fibrillarin. This interaction is essential for several stages in the groundnut rosette virus life cycle such as nucleolar import of the ORF3 proteinviaCajal bodies, relocalization of some fibrillarin from the nucleolus to cytoplasm, and assembly of cytoplasmic umbraviral ribonu-cleoprotein particles that are themselves required for the long-distance spread of the virus and systemic infection. Here, using atomic force microscopy, we determine the architecture of these complexes as single-layered ringlike structures with a diameter of 18-22 nm and a height of 2.0±0.4 nm, which consist of several (n=6-8)distinct protein granules. We also estimate the molar ratio of fibrillarin to ORF3 protein in the complexes as approximately 1:1. Based on these data, we propose a model of the structural organization of fibrillarin-ORF3 protein complexes and discuss potential mechanistic and functional implications that may also apply to other viruses.
650	1	4	_aATOMIC FORCE MICROSCOPY
650	1	4	_aFIBRILLARIN; UMBRAVIRUS
650	1	4	_aVIRUS MOVE-MENT
650	1	4	_aPROTEIN COMPLEXES
700	1	2	_aCanetta, E.
700	1	2	_aKim, S.H.
700	1	2	_aKalinina, N.O.
700	1	2	_aShaw, J.
700	1	2	_aAdya, A.K.
700	1	2	_aGillespie, T.
700	1	2	_aBrown, J.W.S.
700	1	2	_aBrown, J.W.S.
856	4	0	_uhttps://drive.google.com/file/d/1bn11SLCpHOlY1NHLqxvQWi7NbH99Zzy8/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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