000			01906nam a2200241Ia 4500
003			MX-MdCICY
005			20250625153951.0
040			_cCICY
090			_aB-14968
245	1	0	_aNext-Generation Sequencing Platforms
490	0		_vAnnual Review of analytical Chemistry, 6(1), p.287-303, 2013
520	3		_aAutomated DNA sequencing instruments embody an elegant interplay among chemistry, engineering, software, and molecular biology and have built upon Sanger's founding discovery of dideoxynucleotide sequencing to performonce-unfathomable tasks. Combinedwith innovative physical map-ping approaches that helped to establish long-range relationships between cloned stretches of genomic DNA, fluorescent DNA sequencers produced reference genome sequences for model organisms and for the reference hu-man genome. New types of sequencing instruments that permit amazing acceleration of data-collection rates for DNA sequencing have been devel-oped. The ability to generate genome-scale data sets is now transforming the nature of biological inquiry. Here, I provide an historical perspective of the field, focusing on the fundamental developments that predated the ad-vent of next-generation sequencing instruments and providing information about how these instruments work, their application to biological research, and the newest types of sequencers that can extract data from single DNA molecules.
650	1	4	_aMASSIVELY PARALLEL SEQUENCING
650	1	4	_aNEXT-GENERATION SEQUENCING
650	1	4	_aREVERSIBLE DYE TERMINATORS
650	1	4	_aSEQUENCING BY SYNTHESIS
650	1	4	_aSINGLE-MOLECULE SEQUENCING
650	1	4	_aGENOMICS
700	1	2	_aMardis, E.R.
856	4	0	_uhttps://drive.google.com/file/d/1rlvnIKJCX_W9cDgPmu_9jzfZXHJMbrFu/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c49151 _d49151