000			01601nam a2200205Ia 4500
003			MX-MdCICY
005			20250625153952.0
040			_cCICY
090			_aB-15010
245	1	0	_aAn agrobacterium-mediatesd transie4nt gene expression system for intact leaves
490	0		_vPlant Science , 122(1), p.101-108, 1997
520	3		_aAn efficient and reproducible AgrohLlc~teriut,l-mediated transient gene expression system for intact leaf tissue was developed. A high level of transient expression was observed when bacteria. which were pretreated in C?Y gene-induc- ing conditions. were infiltrated into complete leaf tissue. Histochemical /I-glucuronidase assays showed large transgene-expressing sectors comprising of up to 90 percent of the leaf area. As a consequence of infiltration, the induced bacteria entered into the intercellular spaces, thus enabling T-DNA transfer in all cell layers of the leaf. The protocol was optimized for Phasrolus wlgaris leaves. but similar results were obtained with other plant species. such as P/mroh~.s uc,utifdim. poplar, and tobacco. A /I-glucuronidase chimeric gene interrupted by an intron was used as a marker for histological detection of the sectors. Copyright :CJ 1997 Elsevier Science Ireland Ltd.
700	1	2	_aKapila, J.
700	1	2	_aRiet De Rycke,
700	1	2	_aMarc Van Montagu
700	1	2	_aAngenon, G.
856	4	0	_uhttps://drive.google.com/file/d/1I2DtrAD2AaMuCTwLqQMQ0-gdfGCupocE/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c49192 _d49192