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090			_aB-15020
245	1	0	_aCultivation and Preservation of Methanogenic Bacteria
490	0		_vZentralblatt Mikrobiologie , 143(2), p.149-155, 1988
520	3		_aMedium requirements and growth yields of Methanosarcina barkeri, Methanospirillum hungatei, Methanococcus vanielii and Methanobacterium /ormicicum were evaluated. All strains grew well in completely defined media; only M. barkeri required additional vitamins. The maximum achievable biomass yields with H2/C02 (80: 20)as an energy source were 1.5 g dry weight/I. Gas transfer is the limiting factor for growth as was determined in the case of the fermentation of M. vannielii. All strains can be preserved in a viable state either at -20°C or in liquid nitrogen provided that cells from the exponential phase of growth are used. The addition of 2 g cysteine/l to the media results in floculation of M. bryantii and M. /ormicicum.
700	1	2	_aHuber, K.
700	1	2	_aLafferty, R.M.
856	4	0	_uhttps://drive.google.com/file/d/1LK9_ib0BeggS-0XSHxsvmYZzeGuwQYT3/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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