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090 _aB-15038
245 1 0 _aAnalysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis
490 0 _vMethod Mol. Biol. Mol. Tox. Protocols, 1105, p.17-30, 2014
520 3 _aA protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE)is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fl uorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.
650 1 4 _aTWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS
650 1 4 _aTWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
650 1 4 _aPROTEIN SEPARATION
650 1 4 _aPROTEOMICS
700 1 2 _aGao, W.
856 4 0 _uhttps://drive.google.com/file/d/1U6IQUr5SeaUBbagwiD4bqFQJsSFBQ_ho/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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