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245	1	0	_aFructan matabolism in cereals: induction in leaves and compartmentation in protoplasts and vacuoles
490	0		_vZeitschrift fur pflanzenphysiologie, 112(4), p.359-372, 1983
520	3		_aLeaf blades of Triticum aestivum L. cv Kolibri and Hordeum vulgare L. cv Gerbel were induced to accumulate fructan (polyfructosylsucrose of varying molecular size)in large amounts (~70 percent of dwt)by impeding the export of photosynthates. This was achieved by sub­ jecting plants to cold stress (5°C at night)or by continuous illumination of excised leaf blades. Concomitantly with the accumulation of fructan in the leaves the activity of a sucrose­ sucrose-fructosyltransferase (SST), probably the key-enzyme of fructan anabolism, increased several fold in cell-free extracts. Its pH optimum is 5.7. At 8°C its activity is still half of the activity at 28°C, the temperature optimum. This remarkably anomalous dependence on tem­perature is interesting with regard to fructan accumulation in grasses during the cold season. Protoplasts obtained from fructan-enriched barley leaves were employed for the isolation of vacuoles. All the fructans (~trisaccharide)as well as the SST activity were found to be asso­ ciated exclusively with the vacuoles, which therefore appear to play the central role in fructan storage and metabolism.
650	1	4	_aHORDEUM VULGARE
650	1	4	_aTRITICUM AESTIVUM
650	1	4	_aASSIMILATE PARTITIONING
650	1	4	_aCOLD STRESS
650	1	4	_aFRUCTAN
650	1	4	_aPROTOPLASTS
650	1	4	_aVACUOLES
700	1	2	_aWagner, W.
700	1	2	_aKeller, F.
700	1	2	_aWiemken, A.
856	4	0	_uhttps://drive.google.com/file/d/1-aU-vu9zTPa1Ulv1NC15tjiwfaibR90q/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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