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040			_cCICY
090			_aB-15690
245	1	0	_aAsexual Embryogenesis via Callus of Theobroma cacao 1
490	0		_vZ. Pflanzenphysiol., 13, p.347-358., 1984
520	3		_aCallus of Theobroma cacao L. (cacao)possessing embryogenic competence occurred spontaneously with two clones of asexual embryos proliferated in vitro in a hormone-free basal medium by hypocotylary budding. Asexual embryogenesis via callus occurred at low frequency in the hormone-free basal medium. High concentrations of 2,4-D plus coconut water stimulated callus production and suppressed embryo production. Maximum frequency and intensity of embryogenesis occurred at 10-3 to 10-2 mg ·12,4-D. Embryos originated from meristematic tissue at the periphery of callus clumps. During development asexual embryos either remained embedded in the callus or were connected through suspensor-like structures.
650	1	4	_aTHEOBROMA CACAO
650	1	4	_aASEXUAL EMBRYOGENESIS
650	1	4	_aCALLUS
700	1	2	_aKononowicz, H.
700	1	2	_aKononowicz, A.K.
700	1	2	_aJanick, J.
856	4	0	_uhttps://drive.google.com/file/d/1f0CDxpHyWd0jjNQa2yOPuRkk7u6Niqfu/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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