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090			_aB-15708
245	1	0	_aSeparation and characterization of rice proteins
490	0		_vElectrophoresis, 15, p.708-720, 1994
520	3		_aRice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus)were isolated and then separated by two-dimensional gel electrophoresis (2-DE). The protein spots were characterized according to molecular weight, isoelectric point and partial amino-terminal sequence. Electrophoresis was carried out by isoelectric focusing (IEF), nonequilibrium pH gradient electrophoresis (NEPHGE)and immobilized pH gradient (IPG)in the first dimension, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)in the second dimension. With the aid of nine marker proteins, the patterns of IEF, NEPHGE and IPG 2-DE gels were graphically combined by computer into a single synthetic image for each tissue, respectively, and these images for the nine tissues and one organelle were again combined into a single 2-DE image for the integrated rice protein spots. The rice 2-DE gel image resolved 4892 proteins. About 3
700	1	2	_aTsugita, A.
700	1	2	_aKawakami, T.
700	1	2	_aUchiyama, T.
700	1	2	_aKamo, M.
700	1	2	_aMiyatake, M.
700	1	2	_aNozu, N.
856	4	0	_uhttps://drive.google.com/file/d/1Hw_dedTqIhWcC637mliYwKLGwtgqxMZ1/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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