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| 090 | _aB-15926 | ||
| 245 | 1 | 0 | _aA binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid |
| 490 | 0 | _vNature, 303(5913), p.179-180, 1983 | |
| 520 | 3 | _aThe soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown-gall tumours after infection of wounded dicotyledonous plants. Large plasmids (Ti-plasmids)are responsible for the oncogenicity of the bacterium. Crown-gall tumours contain a DNA segment, called the T-DNA, which is homologous with a defined part of the Ti-plasmid present in the tumour-inducing bacterium, and is stably integrated into the plant genome. Apart from the T-DNA another region of the Ti-plasmid, called the vir-region, is essential for tumour induction. We report here the interaction of two compatible plasmids, one containing the vir-region, the other carrying the T-DNA on a wide host-range replicon. An A. tumefaciens strain harbouring both plasmids has a normal tumour-inducing capacity, although neither plasmid is functional alone. With this approach, the T-DNA on one plasmid can, because of its size, be easily genetically manipulated using Escherichia coli as a host. Transfer of this plasmid into an A. tumefaciens strain harbouring the plasmid with the vir-region allows introduction of the manipulated T-DNA into plant cells. In this way, sophisticated binary vector systems for plant genetic engineering can be developed. | |
| 650 | 1 | 4 | _aAGROBACTERIUM TUMEFACIENS |
| 650 | 1 | 4 | _aCARCINOGENICITY |
| 650 | 1 | 4 | _aESCHERICHIA COLI |
| 650 | 1 | 4 | _aGENETIC ENGINEERING |
| 650 | 1 | 4 | _aHEREDITY |
| 650 | 1 | 4 | _aHIGHER PLANT |
| 650 | 1 | 4 | _aINTERACTION |
| 650 | 1 | 4 | _aNONHUMAN |
| 650 | 1 | 4 | _aPLANT |
| 650 | 1 | 4 | _aPLASMID |
| 650 | 1 | 4 | _aSEPARATION |
| 650 | 1 | 4 | _aVECTOR |
| 700 | 1 | 2 | _aHoekema, A. |
| 700 | 1 | 2 | _aHirsch, P.R. |
| 700 | 1 | 2 | _aHooykaas, P.J.J. |
| 700 | 1 | 2 | _aSchilperoort, R.A. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1Z5DHU1kVaHPN4vb4CfZo_2MpmuAoGwc-/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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