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090 _aB-16066
245 1 0 _aQuantitation of proteins in silver stained polyacrylamide gels
490 0 _vFederation Proceedings, 43(6), p.2093, 1984
520 3 _aThis study investigates some of the problems encountered in quantitation of proteins in silver stained polyacrylamide gels. Subunit molecular weight, isoelectric point and amino acid content are examined as possible factors for differential staining intensities of proteins. We also describe a procedure for quantitation using atomic absorption spectroscopy, independent of the colors formed upon silver staining. Using this method we show that some proteins such as pepsin bind silver but fail to develop visual color reactions.
650 1 4 _aPOLYACRYLAMIDE GEL
650 1 4 _aPROTEIN
700 1 2 _aYuksel, K.O.
700 1 2 _aGracy, R.W.
856 4 0 _uhttps://drive.google.com/file/d/1Aiyl-sf_VJPhOZeNSOVuwsSZvIWTYDN5/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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