| 000 | 02342nam a2200253Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625160145.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-16117 | ||
| 245 | 1 | 0 | _aIsoelectric focusing and two-dimensional analysis of purified nitrate reductase from Aspergillus nidulans |
| 490 | 0 | _vBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 706(2), 7, p.203-211, 1982 | |
| 520 | 3 | _aThe assimilatory NADPH-nitrate oxidoreductase (EC 1.6.6.3)from Aspergillus nidulans was purified by means of affinity chromatography and analyzed by agarose isoelectric focusing and two-dimensional electrophoresis. NADPH-nitrate reductase activity was not activated by oxidation with potassium ferricyanide and was irreversibly inhibited by acrylamide. Electrophoresis of nitrate reductase in 7 percent polyacrylamide gels resulted in rapid loss of enzyme activity. Isoelectric focusing of purified enzyme in agarose gels resulted in one homogeneous band that exhibited NADPH-nitrate reductase, NADPH-cytochrome c reductase and reduced methyl viologen-nitrate reductase activities, which corresponded to an isoelectric point of 6.12 ± 0.05 at 22°C. Two-dimensional electrophoresis of focused nitrate reductase on SDS-polyacrylanide gel slabs yielded a single subunit of 54000 molecular weight. Acid treatment of the enzyme and subsequent isoelectric focusing resulted in a protein with a strongly acidic isoelectric point and reduced methyl viologen-nitrate reductase activity. It released another protein with a strongly basic isoelectric point which was inactive. It is postulated that the overall association of fiavoprotein protomers with both heme and cytochrome b1 components confers a small net negative charge upon the native heteromultimer and accounts for its slightly acidic isoelectric point. © 1982. | |
| 650 | 1 | 4 | _aASPERGILLUS NIDULANS |
| 650 | 1 | 4 | _aELECTROPHORESIS, POLYACRYLAMIDE GEL |
| 650 | 1 | 4 | _aISOELECTRIC FOCUSING |
| 650 | 1 | 4 | _aKINETICS |
| 650 | 1 | 4 | _aMOLECULAR WEIGHT |
| 650 | 1 | 4 | _aNITRATE REDUCTASES |
| 700 | 1 | 2 | _aSteiner, F.X. |
| 700 | 1 | 2 | _aDowney, R.J. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/149u9PKXKF81PGGF0Q0d9gef3WnqeEgDp/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 |
_2Loc _cREF1 |
||
| 008 | 250602s9999 xx |||||s2 |||| ||und|d | ||
| 999 |
_c50292 _d50292 |
||