| 000 | 02778nam a2200625Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625160145.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-16123 | ||
| 245 | 1 | 0 | _aOptimized DNA extraction and metagenomic sequencing of airborne microbial communities |
| 490 | 0 | _vNature Protocols, 10(5), p.768-779, 2015 | |
| 520 | 3 | _aMetagenomic sequencing has been widely used for the study of microbial communities from various environments such as soil, ocean, sediment and fresh water. Nonetheless, metagenomic sequencing of microbial communities in the air remains technically challenging, partly owing to the limited mass of collectable atmospheric particulate matter and the low biological content it contains. Here we present an optimized protocol for extracting up to tens of nanograms of airborne microbial genomic DNA from collected particulate matter. With an improved sequencing library preparation protocol, this quantity is sufficient for downstream applications, such as metagenomic sequencing for sampling various genes from the airborne microbial community. The described protocol takes ~ 12 h of bench time over 2-3 d, and it can be performed with standard molecular biology equipment in the laboratory. A modified version of this protocol may also be used for genomic DNA extraction from other environmental samples of limited mass or low biological content. | |
| 650 | 1 | 4 | _aAIR POLLUTION |
| 650 | 1 | 4 | _aAIRBORNE MICROORGANISM |
| 650 | 1 | 4 | _aARTICLE |
| 650 | 1 | 4 | _aCONTROLLED STUDY |
| 650 | 1 | 4 | _aDNA BINDING |
| 650 | 1 | 4 | _aDNA DETERMINATION |
| 650 | 1 | 4 | _aDNA EXTRACTION |
| 650 | 1 | 4 | _aDNA LIBRARY |
| 650 | 1 | 4 | _aDNA SEQUENCE |
| 650 | 1 | 4 | _aGENOME ANALYSIS |
| 650 | 1 | 4 | _aMETAGENOMICS |
| 650 | 1 | 4 | _aMICROBIAL COMMUNITY |
| 650 | 1 | 4 | _aNONHUMAN |
| 650 | 1 | 4 | _aPARTICULATE MATTER |
| 650 | 1 | 4 | _aPRIORITY JOURNAL |
| 650 | 1 | 4 | _aPRIORITY JOURNAL |
| 650 | 1 | 4 | _aSEQUENCE ANALYSIS |
| 650 | 1 | 4 | _aBIOCHEMISTRY |
| 650 | 1 | 4 | _aCHINA |
| 650 | 1 | 4 | _aDEVICES |
| 650 | 1 | 4 | _aDNA SEQUENCE |
| 650 | 1 | 4 | _aENVIRONMENTAL MONITORING |
| 650 | 1 | 4 | _aEQUIPMENT DESIGN |
| 650 | 1 | 4 | _aGENETICS |
| 650 | 1 | 4 | _aISOLATION AND PURIFICATION |
| 650 | 1 | 4 | _aMETAGENOME |
| 650 | 1 | 4 | _aMICROBIAL CONSORTIUM |
| 650 | 1 | 4 | _aMICROBIOLOGY |
| 650 | 1 | 4 | _aPROCEDURES |
| 650 | 1 | 4 | _aQUALITY CONTROL |
| 650 | 1 | 4 | _aWORKFLOW |
| 700 | 1 | 2 | _aJiang, W. |
| 700 | 1 | 2 | _aLiang, P. |
| 700 | 1 | 2 | _aWang, B. |
| 700 | 1 | 2 | _aFang, J. |
| 700 | 1 | 2 | _aLang, J. |
| 700 | 1 | 2 | _aTian, G. |
| 700 | 1 | 2 | _aJiang, J. |
| 700 | 1 | 2 | _aJiang, J. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1gbnvTYtocwx4ee4aF3ZyjdzvqgPOnvjV/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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