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090 _aB-16253
245 1 0 _aStudies on Plant-Pathogen Interactions Using Activity-based Proteomics
490 0 _vCurrent Proteomics, 7(4), p.328-336(9), 2010
520 3 _aTo accelerate functional annotation of proteins with a role during plant-pathogen interactions it is essential to monitor activities of proteins rather than the abundance of transcripts and proteins, since many proteins are posttranslationally regulated during antagonistic interactions. Activity-based protein profiling (ABPP)displays the active proteome using small molecule probes that react with the active site of proteins in an activity-dependent manner. ABPP is a simple and powerful functional proteomics approach that has made important contributions to studies on immune responses and plant-pathogen interactions. ABPP revealed up-regulated proteasome activities during immune responses, and displayed differential serine hydrolase activities of both host and pathogen during infection. Furthermore, ABPP in the presence of putative inhibitors demonstrated that pathogens from different kingdoms produce effectors that suppress different proteolytic activities of the host. Taken together, these examples show that ABPP is a simple and robust way to capture functional information beyond standard proteomic techniques.
650 1 4 _aABPP
650 1 4 _aAVR2
650 1 4 _aACTIVITY-BASED PROTEIN PROFILING
650 1 4 _aBOTRYTIS CINEREA
650 1 4 _aCLADOSPORIUM FULVUM
650 1 4 _aEPICS
650 1 4 _aMES3
650 1 4 _aN-TERMINAL THREONINE
650 1 4 _aPLCP
650 1 4 _aPLCP INHIBITOR E-64
650 1 4 _aPAPAIN-LIKE CYSTEINE PROTEASE
650 1 4 _aPHYTOPHTHORA INFESTANS
650 1 4 _aPROGRAMMED CELL DEATH
650 1 4 _aPSEUDOMONAS SYRINGAE
650 1 4 _aS-FORMYL GLUTATHIONE HYDROLASE
650 1 4 _aS-FORMYL GLUTATHIONE HYDROLASE
650 1 4 _aSERINE HYDROLASES
650 1 4 _aSYLA;ACTIVITY-BASED PROBES
650 1 4 _aCYSTEINE PROTEASE
650 1 4 _aFLUOROPHOSPHONATE
650 1 4 _aMETHYLESTRASES MES2
650 1 4 _aOOMYCETE
650 1 4 _aPATHOGEN EFFECTORS
650 1 4 _aPLANT PATHOGEN INTERACTIONS
650 1 4 _aPROTEASOME
650 1 4 _aSERINE HYDROLASES
650 1 4 _aZYMOGRAMS
700 1 2 _aH. Richau, Kerstin
700 1 2 _aA.L. Van Der Hoorn, Renie
856 4 0 _uhttps://drive.google.com/file/d/19b3pvyyKyZRVDGr7A1TX_5xP_Z8G3CFx/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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