000			02160nam a2200229Ia 4500
003			MX-MdCICY
005			20250625160149.0
040			_cCICY
090			_aB-16302
245	1	0	_aExpression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter
490	0		_vGene, 32(3), p.263-274, 1984
520	3		_aThe promoter region from the cloned glyceraldehyde-3-phosphate dehydrogenase (GPD)gene of Saccharomyces cerevisiae (Musti et al., 1983)has been characterized. A 653-bp TaqI restriction fragment with a 3 ' border 24 bp upstream from the ATG initiation codon was isolated and demonstrated to contain all sequences necessary for promoter function in vivo. This DNA segment was converted to a portable promoter by cloning it into M13mp9, and the entire nucleotide sequence of the portable promoter was determined. Two generalized yeast expression vectors have been constructed utilizing the GPD portable promoter. The expression vectors include the yeast 2/gm origin of replication and amplification functions, such that the plasmids are maintained at high copy number in cir° yeast hosts. These vectors direct synthesis of a consensus ?-interferon(IFN-?Con1)as 1 percent of total cell protein. Hepatitis B surface antigen (HBsAg)was also expressed from these vectors. The 5 ' end of the HBsAg gene was replaced with a synthetic DNA segment which restored the deleted GPD untranslated leader and utilized optimal yeast codons for the first 30 amino acids. The partially synthetic gene resulted in a 10- to 15-fold increased expression level from GPD vectors yielding HBsAg polypeptide as 2-4 percent of total cell protein.
650	1	4	_aALPHA INTERFERON
650	1	4	_aGLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE
650	1	4	_aHEPATITIS B SURFACE ANTIGEN
650	1	4	_aRECOMBINANT DNA
700	1	2	_aBitter, G.A.
700	1	2	_aEgan, K.M.
856	4	0	_uhttps://drive.google.com/file/d/1yG1B_U0VZMwmz3v_u5-CKlajGjzoGkO5/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c50476 _d50476