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090			_aB-16383
245	1	0	_aNaturally occurring off-switches for CRISPR-Cas9
490	0		_vCell, 167(7), p.1829-1838, 2016
520	3		_aCRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that do not employ the Cas9 protein. We posited that nature would also yield Cas9 inhibitors in response to the evolutionary arms race between bacteriophages and their hosts. Here, we report the discovery of three distinct families of anti-CRISPRs that specifically inhibit the CRISPR-Cas9 system of Neisseria meningitidis. We show that these proteins bind directly to N. meningitidis Cas9 (NmeCas9)and can be used as potent inhibitors of genome editing by this system in human cells. These anti-CRISPR proteins now enable "off-switches" for CRISPR-Cas9 activity and provide a genetically encodable means to inhibit CRISPR-Cas9 genome editing in eukaryotes. Video Abstract © 2016 Elsevier
650	1	4	_aANTI-CRISPR
650	1	4	_aCAS9
650	1	4	_aCRISPR-CAS
650	1	4	_aGENOME EDITING
650	1	4	_aNEISSERIA MENINGITIDIS
650	1	4	_aPHAGE
700	1	2	_aPawluk, A.
700	1	2	_aAmrani, N.
700	1	2	_aZhang, Y.
700	1	2	_aGarcia, B.
700	1	2	_aHidalgo-Reyes, Y.
700	1	2	_aLee, J.
700	1	2	_aEdraki, A.
700	1	2	_aEdraki, A.
700	1	2	_aSontheimer, E.J.
700	1	2	_aMaxwell, K.L.
700	1	2	_aDavidson, A.R.
856	4	0	_uhttps://drive.google.com/file/d/1Yak_VkTUJRGaA86vR2NMZKw53fbnCkFS/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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