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090 _aB-16437
245 1 0 _aMaintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis
490 0 _vNature Genetics, 34(1), p.65-69, 2003
520 3 _aIn mammals, the DNA methyltransferase 1 (Dnmt1)faithfully copies the pattern of cytosine methylation at CpG sites to the newly synthesized strand, and this is essential for epigenetic inheritance1. In Arabidopsis thaliana, several DNA methyltransferases or chromatin modifiers coupled to methylation changes have been characterized, and mutations that cause loss of their function are recessive2-7. This is surprising because plant gametogenesis includes postmeiotic DNA replication in haploid nuclei before fertilization. Therefore, the recessive character of the mutations excludes the affected components from a regulatory role in postmeiotic maintenance or modification of epigenetic states. Here we show, however, that depletion of A. thaliana MET1, a homolog of mammalian Dnmt1 (ref. 8), results in immense epigenetic diversification of gametes. This diversity seems to be a consequence of passive postmeiotic demethylation, leading to gametes with fully demethylated and hemidemethylated DNA, followed by remethylation of hemimethylated templates once MET1 is again supplied in a zygote.
650 1 4 _aARABIDOPSIS
650 1 4 _aARTICLE
650 1 4 _aCHROMATIN
650 1 4 _aCONTROLLED STUDY
650 1 4 _aCPG ISLAND
650 1 4 _aDEMETHYLATION
650 1 4 _aDNA METHYLATION
650 1 4 _aDNA MODIFICATION
650 1 4 _aDNA REPLICATION
650 1 4 _aFERTILIZATION
650 1 4 _aGAMETOGENESIS
650 1 4 _aGENE FUNCTION
650 1 4 _aGENE MUTATION
650 1 4 _aGENETIC VARIABILITY
650 1 4 _aHAPLOIDY
650 1 4 _aHAPLOIDY
650 1 4 _aMEIOSIS
650 1 4 _aNONHUMAN
650 1 4 _aNUCLEOTIDE SEQUENCE
650 1 4 _aPRIORITY JOURNAL
650 1 4 _aPROTEIN SYNTHESIS
650 1 4 _aRECESSIVE INHERITANCE
650 1 4 _aSEQUENCE ANALYSIS
650 1 4 _aSEQUENCE HOMOLOGY
650 1 4 _aZYGOTE
700 1 2 _aSaze, H.
700 1 2 _aScheid, O.M.
700 1 2 _aPaszkowski, J.
856 4 0 _uhttps://drive.google.com/file/d/1oFmmyXEV4XcpJOVmL7LWZIrVZ_j87elg/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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