| 000 | 01582nam a2200265Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625160152.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-16472 | ||
| 245 | 1 | 0 | _aQuantitative Auxin Metabolite Profiling Using Stable Isotope Dilution UHPLC-MS/MS |
| 490 | 0 | _vCurrent Protocols in Plant Biology, DOI: 10.1002/cppb.20028, 2016 | |
| 520 | 3 | _aAuxin, a plant hormone, is able to stimulate growth and to control developmental processes. To get a deeper level of understanding of how auxin activity is regulated in planta, we have developed a sensitive and selective mass spectrometry-based protocol for multiplex quantification of indole 3-acetic acid (IAA)as well as its precursors and degradation products. As part of the protocol development, we have also established a derivatization protocol for the IAA precursors IPyA and IAAld, compounds that are highly labile during sample extraction and purification. This article describes a standard isotope dilution method for quantitative profiling of the IAA metabolome in small amounts of plant tissues. © 2016 by John Wiley & Sons, Inc. | |
| 650 | 1 | 4 | _aAUXIN |
| 650 | 1 | 4 | _aIAA |
| 650 | 1 | 4 | _aLC-MS/MS |
| 650 | 1 | 4 | _aMETABOLITE PROFILING |
| 650 | 1 | 4 | _aSPE |
| 700 | 1 | 2 | _aNovák, Ondrej |
| 700 | 1 | 2 | _aPencík, Ale |
| 700 | 1 | 2 | _aBlahousek, Ota |
| 700 | 1 | 2 | _aLjung, Karin |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1gg4LE37umEQ0REGW86QL2zGjeDVJmyVz/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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