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040			_cCICY
090			_aB-16601
245	1	0	_aRapid cDNA sequencing (expressed sequence tags)from a directionally cloned human infant brain cDNA library
490	0		_vNat Genet., 4(4), p.373-380, 1993
520	3		_aA human infant brain cDNA library, made specifically for production of expressed sequence tags (ESTs)was evaluated by partial sequencing of over 1,600 clones. Advantages of this library, constructed for EST sequencing, include the use of directional cloning, size selection, very low numbers of mitochondrial and ribosomal transcripts, short polyA tails, few non-recombinants and a broad representation of transcripts. 37 percent of the clones were identified, based on matches to over 320 different genes in the public databases. Of these, two proteins similar to the Alzheimer's disease amyloid precursor protein were identified.
650	1	4	_aCDNA SEQUENCING
700	1	2	_aAdams Md
700	1	2	_aSoares Mb
700	1	2	_aKerlavage Ar
700	1	2	_aFields C
700	1	2	_aVenter Jc.
856	4	0	_uhttps://drive.google.com/file/d/1t7ffkVONi5IEsc9aNcPt5bLnntB-D8Vq/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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