| 000 | 03527nam a2200373Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625160155.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-16638 | ||
| 245 | 1 | 0 | _aThe Microphenotron: A robotic miniaturized plant phenotyping platform with diverse applications in chemical biology |
| 490 | 0 | _vPlant Methods, 13(1), p.Article number 10, 2017 | |
| 520 | 3 | _aBackground: Chemical genetics provides a powerful alternative to conventional genetics for understanding gene function. However, its application to plants has been limited by the lack of a technology that allows detailed phenotyping of whole-seedling development in the context of a high-throughput chemical screen. We have therefore sought to develop an automated micro-phenotyping platform that would allow both root and shoot development to be monitored under conditions where the phenotypic effects of large numbers of small molecules can be assessed. Results: The 'Microphenotron' platform uses 96-well microtitre plates to deliver chemical treatments to seedlings of Arabidopsis thaliana L. and is based around four components: (a)the 'Phytostrip', a novel seedling growth device that enables chemical treatments to be combined with the automated capture of images of developing roots and shoots; (b)an illuminated robotic platform that uses a commercially available robotic manipulator to capture images of developing shoots and roots; (c)software to control the sequence of robotic movements and integrate these with the image capture process; (d)purpose-made image analysis software for automated extraction of quantitative phenotypic data. Imaging of each plate (representing 80 separate assays)takes 4 min and can easily be performed daily for time-course studies. As currently configured, the Microphenotron has a capacity of 54 microtitre plates in a growth room footprint of 2.1 m2, giving a potential throughput of up to 4320 chemical treatments in a typical 10 days experiment. The Microphenotron has been validated by using it to screen a collection of 800 natural compounds for qualitative effects on root development and to perform a quantitative analysis of the effects of a range of concentrations of nitrate and ammonium on seedling development. Conclusions: The Microphenotron is an automated screening platform that for the first time is able to combine large numbers of individual chemical treatments with a detailed analysis of whole-seedling development, and particularly root system development. The Microphenotron should provide a powerful new tool for chemical genetics and for wider chemical biology applications, including the development of natural and synthetic chemical products for improved agricultural sustainability. | |
| 650 | 1 | 4 | _aARABIDOPSIS THALIANA |
| 650 | 1 | 4 | _aAUTOMATED |
| 650 | 1 | 4 | _aBIOSTIMULANTS |
| 650 | 1 | 4 | _aCHEMICAL BIOLOGY |
| 650 | 1 | 4 | _aCHEMICAL GENETICS |
| 650 | 1 | 4 | _aERAGROSTIS TEF |
| 650 | 1 | 4 | _aPLANT PHENOTYPING |
| 650 | 1 | 4 | _aROBOTIC |
| 650 | 1 | 4 | _aROOT SYSTEM ARCHITECTURE |
| 650 | 1 | 4 | _aSHOOT DEVELOPMENT |
| 700 | 1 | 2 | _aBurrell, T. |
| 700 | 1 | 2 | _aFozard, S. |
| 700 | 1 | 2 | _aHolroyd, G.H. |
| 700 | 1 | 2 | _aFrench, A.P. |
| 700 | 1 | 2 | _aPound, M.P. |
| 700 | 1 | 2 | _aBigley, C.J. |
| 700 | 1 | 2 | _aJames Taylor, C. |
| 700 | 1 | 2 | _aJames Taylor, C. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1q4l0LPWl2-JBrqnDG1PMxdUd4ZgtwjgL/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 |
_2Loc _cREF1 |
||
| 008 | 250602s9999 xx |||||s2 |||| ||und|d | ||
| 999 |
_c50808 _d50808 |
||