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245	1	0	_aLipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator
490	0		_vNat Biotechnol., 35(7), p.647-652, 2017
520	3		_aLipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation. Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors. Knocking out a homolog of fungal Zn(II)2Cys6-encoding genes improved partitioning of total carbon to lipids from 20
700	1	2	_aAjjawi I
700	1	2	_aVerruto J
700	1	2	_aAqui M
700	1	2	_aSoriaga Lb
700	1	2	_aCoppersmith J
700	1	2	_aKwok K
700	1	2	_aPeach L
700	1	2	_aPeach L
700	1	2	_aKalb R
700	1	2	_aXu W
700	1	2	_aCarlson Tj
700	1	2	_aFrancis K
700	1	2	_aKonigsfeld K
700	1	2	_aBartalis J
700	1	2	_aSchultz A
700	1	2	_aLambert W
700	1	2	_aSchwartz As
700	1	2	_aBrown R
700	1	2	_aMoellering Er
856	4	0	_uhttps://drive.google.com/file/d/177Tkzk9POWsmWmuyQaWcnyH1GlABdf5m/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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