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090			_aB-16668
245	1	0	_aGrowth, Cytology and Flow Cytometry of Embryogenic Cell Suspension Cultures of Panicum maximum Jacq. and Pennisetum purpureum Schum.
490	0		_vJournal of Plant Physiology, 123(3), p.211-227, 1986
520	3		_aEmbryogenic cell suspensions of Panicum maximum (4 year old)and Pennisetum purpureum (3 year old)continue rapid growth upon subculture without a marked lag phase. They have cell doubling times of 27 h and 32 h, respectively, during the exponential phase of growth. Maximum cell numbers of 2 × 10? cells · ml-1 are reached on about the eighth day of subculture in both species. Thereafter, cell lysis and death occur progressively. By day 16, 90 percent of P. purpureum cells appear non-viable. Flow cytometry of the cell suspensions showed that at any given time during the 8-9 day culture period, a majority of the cells are in G1 phase of the cell cycle, with substantial numbers cycling through the S and G2 phases. No marked change in this distribution pattern was seen in the first 8-9 days of growth during which the cultures did not reach stationary phase. Flow cytometry, microspectrophotometry and chromosome counts showed the cultures comprised predominantly ofnormal diploid cells.
650	1	4	_a2,4-D
650	1	4	_a2,4-DICHLOROPHENOXYACETIC ACID
650	1	4	_aCELL CYCLE
650	1	4	_aCELL SUSPENSION CULTURE
650	1	4	_aCEREALS
650	1	4	_aDRY WEIGHT
650	1	4	_aDW
650	1	4	_aFLOW CYTOMETRY
650	1	4	_aFRESH WEIGHT
650	1	4	_aFW
650	1	4	_aGRASSES
650	1	4	_aMICROSPECTROPHOTOMETRY
650	1	4	_aPACKED CELL VOLUMEN
650	1	4	_aPANICUM MÁXIMUM
650	1	4	_aPCV
650	1	4	_aPCV
650	1	4	_aSOMATIC EMBRYOGENESIS
650	1	4	_aTISSUE CULTURE
700	1	2	_aKarlsson, S.B.
700	1	2	_aVasil, I.K.
856	4	0	_uhttps://drive.google.com/file/d/1XsHxI796IuBZUMdFkeRZk8l94sdZbd-s/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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