000			02730nam a2200253Ia 4500
003			MX-MdCICY
005			20250625160156.0
040			_cCICY
090			_aB-16704
245	1	0	_aCharacterization of causal agents of a novel disease inducing brown-black spots on tender tea leaves in China
490	0		_vPlant Disease, 101(10), p.1802-1811, 2017
520	3		_aA novel disease characterized by small brown-black spots (1 to 2 mm in diameter)on tender tea leaves (Camellia sinensis)has been observed in many regions of Hubei Province, China, which severely affects the yield and quality of tea. Tea leaf samples with typical symptoms were collected from three major tea-cultivation regions of Hubei, and were subjected to pathogen isolation for etiological analysis. As a result, 34 Pestalotiopsis isolates were obtained from 20 samples, and they were identified as Pestalotiopsis theae (14 isolates), P. camelliae (12), and P. clavispora (8), determined by morphologies and phylogenetic analysis based on internal transcribed spacer, and partial ß-tubulin and translation elongation factor 1-alpha genes. Pathogenicity tests on detached tea leaves showed that no matter what mycelial discs or conidium suspensions were used, inoculation of the Pestalotiopsis fungi could result in small brown-black spots (1 to 2 mm in diameter)on wounded leaves, similar to those observed in the field in the sizes and colors. It also revealed that only P. theae had pathogenicity on unwounded tea leaves, and P. theae and P. clavispora showed significantly higher virulence than P. camelliae. Inoculation test with conidium suspension on intact tea leaves in the field further confirmed that P. theae as the pathogen of brown-black spots. Reisolation of the pathogens from diseased leaves confirmed that the symptom was caused by the inoculation of Pestalotiopsis fungi. The P. theae isolates responsible for brown-black spots were also compared with those for tea gray blight disease in growth rate, pathogenicity, and molecular characteristics in parallel. To our knowledge, this is the first report that the Pestalotiopsis fungi cause brown-black spot disease on tender tea leaves. The results provide important implications for the prevention and management of this economically important disease.
650	1	4	_aTEA LEAVES
700	1	2	_aWang, Z.H.
700	1	2	_aZhao, Z.X.
700	1	2	_aHong, N.
700	1	2	_aNi, D.
700	1	2	_aCai, L.
700	1	2	_aXu, W.X.
700	1	2	_aXiao, Y.N.
856	4	0	_uhttps://drive.google.com/file/d/10jkJmOUvAOVyYb4vIGKw1RVtfG5dCCpL/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c50874 _d50874