000			01892nam a2200301Ia 4500
003			MX-MdCICY
005			20250625160156.0
040			_cCICY
090			_aB-16725
245	1	0	_aCRISPR/Cas9-Based Engineering of the Epigenome
490	0		_vCell Stem Cell, 21(4), p.431-447, 2017
520	3		_aDetermining causal relationships between distinct chromatin features and gene expression, and ultimately cell behavior, remains a major challenge. Recent developments in targetable epigenome-editing tools enable us to assign direct transcriptional and functional consequences to locus-specific chromatin modifications. This Protocol Review discusses the unprecedented opportunity that CRISPR/Cas9 technology offers for investigating and manipulating the epigenome to facilitate further understanding of stem cell biology and engineering of stem cells for therapeutic applications. We also provide technical considerations for standardization and further improvement of the CRISPR/Cas9-based tools to engineer the epigenome. Pulecio et al. discuss opportunities that CRISPR/Cas9 technology offers for investigating and manipulating the stem cell epigenome and also provide technical considerations for CRISPR/Cas9 tool standardization and improvement.
650	1	4	_aCHROMATIN ARCHITECTURE
650	1	4	_aCRISPR-DCAS9
650	1	4	_aDNA METHYLATION
650	1	4	_aHISTONE MODIFICATIONS
650	1	4	_aTARGETED EPIGENOME ENGINEERING
650	1	4	_aTARGETED GENE EDITION
650	1	4	_aTRANSCRIPTIONAL REGULATION
700	1	2	_aPulecio, J.
700	1	2	_aVerma, N.
700	1	2	_aMejía-Ramírez, E.
700	1	2	_aHuangfu, D.
700	1	2	_aRaya, A.
856	4	0	_uhttps://drive.google.com/file/d/1l-BWzjRzu5kw0dUsVT6H0tBDuwqkBe6Y/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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