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090 _aB-16779
245 1 0 _aCRISPR History: Discovery, Characterization, and Prosperity
490 0 _vProgress in Molecular Biology and Translational Science, 152, p.1-21, 2017
520 3 _aCRISPR research is a very young research field since it was only 10. years ago when the system was found to confer antiviral defense. Nevertheless, there has been an explosion of publications in CRISPR research in the past 5. years. The research was started with the comparative genomics of microbial genomes early this century, which revealed the prevalence of clustered regularly interspaced short palindromic repeats (CRISPR)and CRISPR-associated (Cas)in bacteria and archaea. Series of hypotheses were made based on bioinformatics analyses and tested experimentally within a few years after the CRISPR acronym was coined. These findings have not only led to the discovery of the unique antiviral system and the involved molecular mechanisms, but also to the development of CRISPR technology with various well-developed applications, such as genome editing in all three domains of life. Currently, widespread research efforts in multiple research disciplines have constantly yielded new insights into molecular mechanisms of CRISPR antiviral immunity, and new applications in scientific research and biomedical applications. Retrospectively, it is worth pointing out that close interdisciplinary interactions have fostered series of discoveries in the CRISPR research and worked as the driving force in the fast developing research field.
650 1 4 _aANTI-CRISPR SYSTEMS
650 1 4 _aCAS GENE CASSETTES
650 1 4 _aCASCADE
650 1 4 _aCRISPR LOCI
650 1 4 _aCRISPR TECHNOLOGY
650 1 4 _aCRRNA BIOGÉNESIS
650 1 4 _aDNA INTERFERENCE
650 1 4 _aGRNA-CAS9
650 1 4 _aRNP
650 1 4 _aSPACER ADAPTATION
700 1 2 _aHan, W.
700 1 2 _aShe, Q.
856 4 0 _uhttps://drive.google.com/file/d/1wFHyF4FwBg85v2htx7jVFwFUyV5iqxSn/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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