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003			MX-MdCICY
005			20250625160200.0
040			_cCICY
090			_aB-16907
245	1	0	_aSecuenciación del genoma completo del potato yellow vein virus (PYVV)en tomate (solanum lycopersicum)en Colombia = Genome sequencing of potato yellow vein virus (PYVV)strain infecting solanum lycopersicum in Colombia
490	0		_vActa Biologica Colombiana, 22(1), p.5-17, 2017
520	3		_aPotato yellow vein virus (PYVV)is one of the most important pathogens of potato in the Andean region. In spite of having been detected in tomato crops in Colombia, knowledge on the biological characteristics of PYVV is limited on this host. In this study, next-generation sequencing (NGS)of a PYVV strain infecting tomato in Marinilla District (Antioquia)was performed; additionally, three primer set useful in RT-PCR and RT-qPCR detection were also tested. The consensus genome consisted of three RNA segments of 8043 nt (RNA1), 5346 nt (RNA2)and 3896 nt (RNA3)encoding ten ORF with slight lower sequence identity in relation to PYVV isolates from potato. Sequence analysis suggests the presence of regions potentially undergoing positive selection in the ORFs coding for MET/HEL and CPm possibly as a result of host adaptation. Experimental validation of primers resulted in amplicon with the expected size while melting temperature analysis and sequencing suggest the presence of at least two PYVV infecting S. lycopersicum in east Antioquia in agreement with the NGS data. © 2016, Universidad Nacional de Colombia
650	1	4	_aCRINIVIRUS
650	1	4	_aHIGH-THROUGHPUT NUCLEOTIDE SEQUENCING
650	1	4	_aRT-PCR
650	1	4	_aRT-QPCR
650	1	4	_aSOLANACEAE
700	1	2	_aMuñoz Baena, L.
700	1	2	_aGutiérrez Sánchez, P.A
700	1	2	_aMarín Montoya, M.
856	4	0	_uhttps://drive.google.com/file/d/1ZZduCpIJ8mIAoQR7y87CzChBqJjTsH6a/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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