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090 _aB-16919
245 1 0 _aProduction of transgenic sugarcane (Saccharum officinarum L.)plants by intact cell electroporation
490 0 _vPlant Cell Reports, 14(5):305-309, 1995
520 3 _aWe describe an efficient procedure for genetic transformation of commercial sugarcane varieties POJ 2878 and Ja 60-5. The transformation protocol is based on electroporation of a plasmid conferring GUS activity into cell clusters isolated from embryogenic calli. Six to eight weeks after electroporation, Ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and Southern hybridization analysis. Electroporation of intact cells is an efficient and reproducible method for sugarcane transformation and may also be useful for transformation of other plants.
650 1 4 _aSUGARCANE
650 1 4 _aELECTROPORATION
650 1 4 _aGUS
650 1 4 _aSTABLE TRANSFORMATION
700 1 2 _aArencibia A
700 1 2 _aMolina Pr
700 1 2 _aDe La Riva G
700 1 2 _aSelman-Housein G.
856 4 0 _uhttps://drive.google.com/file/d/10js3hkezESnJBZiQ8eeEtuiLXhP5xWMj/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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