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090			_aB-16926
245	1	0	_aSomatic embryogenesis in Carica papaya through zygotic embryo derived callus culture
490	0		_vActa Horticulturae, 851, p.201-208, 2010
520	3		_aA rapid and efficient system for papaya (Carica papaya L. cv. Co7)regeneration through indirect somatic embryogenesis has been established. Embryogenic calli were obtained from zygotic embryos (excised from 110-120 dayold fruits)on CIM [half-strength MS (Murashige and Skoog, 1962)basal salts and vitamins, 400 mg/L of glutamine, 6 percent sucrose, 4 g/L phytagel]supplemented with varying concentrations 0.5-12.0 mg/L of 2,4-D. The frequency of somatic embryo formation was as high as 54.35 percent when cultured on medium containing 2.0 mg/L of 2,4-D. Suspension cultures were superior to static cultures (solid medium)for the maturation of somatic embryos. Matured (cotyledonary stage)somatic embryos cultured on MSR medium supplemented with 0.4 mg/L BAP and 0.02 mg/L NAA showed regeneration efficiency up to 62.32 percent. The emerging shoots with 2-3 trilobed leaves were rooted on MSL medium containing 1.0 mg/L of IBA with efficiency of 60.07 percent. Plants with well-developed shoots and roots were subsequently hardened on a potting mixture enriched with arbuscular mycorrhiza. The plantlets from zygotic embryo explants were morphologically similar and normal. No phenotypic variations have been observed in field-established plants. This regeneration protocol assures a high frequency of somatic embryogenesis, maturation and regeneration into plantlets.
650	1	4	_aPAPAYA
650	1	4	_aSOMATIC EMBRYOS
650	1	4	_aZYGOTIC EMBRYOS
700	1	2	_aRamaswamy, A.
700	1	2	_aPhap, P.D.
700	1	2	_aSoorianathasundaram, K.
700	1	2	_aKumar, N.
700	1	2	_aThirugnanakumar, S.
700	1	2	_aSudhakar, D.
700	1	2	_aBalasubramanian, P.
856	4	0	_uhttps://drive.google.com/file/d/1IzO75rjKI3GzqcSJQTI886nqmTQtFE88/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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