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245	1	0	_aIn vitro growth of Melaleuca alternifolia Cheel in bioreactor of immersion by bubbles
490	0		_vPakistan Journal of Botany, 43(6), p.2937-2939, 2011
520	3		_aDevelopment of new techniques for in vitro culture is necessary to optimize and reduce the production costs. The aim of this work was to introduce the bioreactor BIB® (Bioreactor of Immersion by Bubbles)and compare it with conventional culture and bioreactor RITA® (Recipient with Automatized Temporary Immersion)for In vitro growth of M. alternifolia plantlets. Plantlets with 1.0 cm height from the fifth In vitro subculture were used. Treatments were: (1)conventional culture (control)using 200 mL glass flasks covered with polypropylene lids; (2)bioreactor RITA® and (3)bioreactor BIB®. Continuous immersion and aeration was used in bioreactors. It was used liquid MS medium supplemented with 30 g.L-1 sucrose and 1 drop.200 mL-1 Tween 20® (i.e. detergent for bubbles formation). BIB® obtained best results for total fresh mass (0.39 g)and shoot fresh mass (0.27 g), fresh mass increment (3.1), total dry mass (0.036 g)and shoot dry mass (0.033 g)than RITA® and conventional culture. In conclusion, BIB® is more efficient to the biomass accumulation of M. alternifolia plantlets.
650	1	4	_aMELALEUCA
650	1	4	_aMELALEUCA ALTERNIFOLIA
700	1	2	_aScheidt, G.N.
700	1	2	_aDa Silva, A.L.L.
700	1	2	_aDe Oliveira, Y.
700	1	2	_aDa Luz Costa, J.
700	1	2	_aBiasi, L.A.
700	1	2	_aSoccol, C.R.
856	4	0	_uhttps://drive.google.com/file/d/13UVRQ-HhZUa1uCANcXLkqAjn8blfnRdR/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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